Generation of Reactive Oxygen Species and Free Electrons in Visible Light Illuminated Cells and Plasma Membranes
نویسندگان
چکیده
Visible light has been used successfully over the last three decades for biostimulation of various tissues. Applications include stimulation of wound healing improving fertilizing and more. Previously, we suggested that low levels of reactive oxygen species (ROS) serve as messengers in light mediated stimulation. Using the EPR spin trapping coupled with the probe, we succeeded to detect ROS generation in isolated cells and sperm membranes illuminated with broad band visible light. We found that low energy visible light (LEVL) not only generates O2, •OH and O2• ̄ but might also enhances other radicals such as, phenyl and free electron. Our results also suggest that the membrane components NADPH oxidase system participate in ROS production by LEVL.
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